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KMID : 0948320070070020079
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Konyang Medical Journal
2007 Volume.7 No. 2 p.79 ~ p.83
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Effect of Medicinal Plant Extracts on the Expression of Collagen and Matrix Metalloproteinase-1 in Human Skin Fibroblasts
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Sim Gwan-Sub
Cho Young-Ho
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Abstract
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The production of matrix metalloproteinases (MMPs) by the UV irradiated skin fibroblast and the degradation of extracellular matrix (ECM) by these enzymes is known as one of the main reasons of photoaging. In the present study, to develop a new anti-wrinkle agent, we have investigated the effects of medicinal plant extracts on collagen synthesis and MMP-1 expression in the human dermal fibroblasts. Among the extracts from medicinal plants, the Selaginella tamariscina extract was found to exhibit a significant effect on type I collagen synthesis and inhibition of MMP-1 expression. The results from enzyme-linked immunosorbent assay (ELISA) and reverse transcriptasepolymerase chain reaction (RT-PCR) showed that the S. tamariscina extract treatment increased the synthesis of type I collagen by 31.0±3.0% and decreased the expression of UVA-induced MMP-1 by 57.0±3.0%. To further investigate the precise involvement of the S. tamariscina extract, the changes in mRNA expression of the type I collagen and MMP-1 were studied by using the RT-PCR techniques. The results showed that the S. tamariscina extract did not make any change of the mRNA expression of type I collagen, while, it decreased the mRNA expression of MMP-1. Therefore, the S. tamariscina extract was able to significantly increase collagen synthesis in the protein level and decrease UVA-induced MMP-1 expression at the protein level and mRNA level. These results demonstrated that the S. tamariscina extract could be a candidate agent for a photoaging by increasing collagen synthesis and reducing UVA-induced MMP-1 expression.
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KEYWORD
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Medicinal plants, Photoaging, Collagen, MMP-1, RT-PCR
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